Culture Supernatants of <I>Lactobacillus acidophilus</I> and <I>Bifidobacterium adolescentis</I> Repress Ileal Ulcer Formation in Rats Treated with a Nonsteroidal Antiinflammatory Drug by Suppressing Unbalanced Growth of Aerobic Bacteria and Lipid Peroxidation

J-STAGE
  • Kinouchi Takemi
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Kataoka Keiko
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Bing Shan Ruo
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Nakayama Haruyuki
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Uejima Motoo
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Shimono Kazuyuki
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Kuwahara Tomomi
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Akimoto Shigeru
    Depament of Bacteriology, School of Medicine, The University of Tokushima
  • Hiraoka Isao
    Naruto Research Institute, Otsuka Pharmaceutical Factory, Inc.
  • Ohnishi Yoshinari
    Depament of Bacteriology, School of Medicine, The University of Tokushima

抄録

A nonsteroidal antiinflammatory drug, 5-bromo-2- (4-fluoropheny1) -3- (4-methylsulfonylphenyl) thiophene (BFMeT), induced ileal ulcers in rats after oral administration, while no ulcers were observed after subcutaneous injection. The ileal ulcer formation in BFMeT-treated rats was examined to correlate the administration of cultures of Lactobacillus acidophilus or Bifidobacterium adolescentis with intestinal bacteria in the ileal contents and lipid peroxidation of the small intestinal mucosa. Ileal ulcers were observed in more than 85% of the rats treated with BFMeT at a dose of 1, 000 mg/kg when they were given tap water as drinking water. The incidence of ulcer formation was repressed by giving culture supernatants of L. acidophilus or B. adolescentis as drinking water, but not by giving the cell suspension as drinking water. Gram staining of the ileal contents of normal rats revealed that 97 % of the stained bacteria were Gram-pos itive rods and only 1.5% were Gram-negative rods. The percentage of Gram-negative rods 72 hr after BFMeT administration was 49.8% and increased over 30-fold in BFMeT-treated rats. However, the percentage of Gram-negative rods was 9.7% or 16%, respectively, in rats taking culture supernatants of L. acidophilus or B. adolescentis. In addition, thiobarbituric acid-reactive substances in the ileal mucosa increased significantly in the rats given tap water for 72 hr after BFMeT treatment, but not in rats given the culture supernatants of L. acidophilus or B. adolescentis. Since BFMeT induced an unbalanced intestinal microflora, the effect of antibiotic treatment on ulcer formation in rats was examined. The magnitude of the ulcer formation in the antibiotic-treated rats was, in decreasing order, metronidazole > none > kanamycin > a mixture (bacitracin, neomycin and streptomycin). These results suggest that the intestinal microflora plays an important role in ulcer formation and that a metabolite (s) of L. acidophilus and B. adolescentis inhibits ileal ulcer formation by repressing changes in the intestinal microflora and lipid peroxi dation in BFMeT-treated rats.

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