Rapid Analysis of Allelic Variants of the Sheep PrP Gene by Oligonucleotide Probes

• Ishiguro Naotaka

  Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine

• Shinagawa Morikazu

  Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine

• Onoe Sadao

  Takikawa Animal Husbandry Experiment Station

• Yamanouchi Kazunori

  Takikawa Animal Husbandry Experiment Station

• Saito Toshiro

  Takikawa Animal Husbandry Experiment Station

抄録

A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with ³²P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP^MARQ PrP^TARQ and PrP^MARR, were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171-Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie-resistant progenitors in the flocks.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 42 (8), 579-582, 1998

  Center For Academic Publications Japan

詳細情報

• CRID: 1571980078012267392
• NII論文ID: 130003484630
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00085213883?from=CiNii
• 本文言語コード: en
• データソース種別:
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