Anti-HIV-1 Activity of an Ionically Modified Porous Polypropylene Membrane Determined by Filtration of a Viral Suspension
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- Owada Takashi
- Department of New Materials Section, Terumo Research and Development Center
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- Miyashita Yuko
- Department of New Materials Section, Terumo Research and Development Center
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- Motomura Tadahiro
- Department of New Materials Section, Terumo Research and Development Center
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- Onishi Makoto
- Department of New Materials Section, Terumo Research and Development Center
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- Yamashita Shuzo
- Department of New Materials Section, Terumo Research and Development Center
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- Yamamoto Naoki
- Department of Microbiology, Tokyo Medical and Dental University School of Medicine
抄録
We describe here a unique anti-HIV-1 membrane, derived from a chemically modified porous polypropylene (PP) membrane, which lowers viral infectivity upon the filtration of HIV-1 suspension. A cationic polymer, polyethyleneimine (PEI) was graft-polymerized onto the PP filter membrane (PP-PEI), and infectious HIV-1HTLV-IIIB derived from MOLT-4/HIV-1HTLV-IIIB cells (HIV-1HTLV-IIIB(MOLT-4)) was applied. When a viral suspension of high titer (103.93 TCID50 ml-1) was filtered, efficient reduction (>99%) of gag p24 antigen levels and infectious titer resulted. In a viral suspension of medium titer (102.37 TCID50 ml-1), a significant decrease in the p24 antigen did not occur, although the titer was markedly reduced (>95%). Electron microscopic observation suggested that PEI induced viral aggregations under high titer conditions, and under medium titer conditions, PEI deprived HIV-1HTLV-IIIB of its infectivity alone to avoid virus adsorption. In contrast, HIV-1 propagated in human peripheral blood mononuclear cells (PBMC) such as HIV-1HTLV- IIIB(PBMC) was more efficiently trapped by PP-PEI at lower titers as compared with HIV-1HTLV-IIIB(MOLT-4) from MOLT-4/HIV-1HTLV-IIIB cells. These data suggest host cell modification in the interactions between PP-PEI and HIV-1 strains. Since HIV-1HTLV-IIIB(MOLT-4) and HIV-1HTLV-IIIB(PBMC) were almost electrically neutral and negative, respectively, we concluded that the divergent effect of PEI on each HIV-1HTLV-IIIB resulted from their different electrical characteristics.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 43 (2), 141-151, 1999
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1570291228151984384
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- NII論文ID
- 130003484676
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles