High Molecular Weight Dextran Sulfate Increases the Activity of NF-κB-Regulated Promoter in Monocyte-Derived Macrophages

• Jagodzinski Pawel P.

  Department of Physiological Chemistry, University of Medical Sciences

• Trzeciak Wieslaw H.

  Department of Physiological Chemistry, University of Medical Sciences

抄録

It is known that sulfated polysaccharides can mimic the action of common T-cell mitogens. To investigate the molecular basis of the mitogenic effect of high molecular weight dextran sulfate (HMDS), monocyte-derived macrophages were transfected with recombinant plasmid containing chloramphenicol acetyl transferase (CAT) reporter gene under the control of the HIV-1 long terminal repeat (LTR) promoter, which is regulated by transcription factor NF-κB. We observed that HMDS, similar to bacterial lipopolysaccharide (LPS), increases the expression of CAT reporter gene suggesting increased activity of NF-κB. The activation of NF-κB correlated with the increased expression of B7.1 molecules. It was postulated that this NF-κB-regulated promoter might play a role in the activation of the accessory cells as well as the rate of replication of HIV-1 in monocyte-derived macrophages.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 43 (2), 187-189, 1999

  Center For Academic Publications Japan

詳細情報

• CRID: 1573950402849236608
• NII論文ID: 130003484683
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00085862586?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles