The Majority of Lymphocytes in the Bone Marrow, Thymus and Extrathymic T Cells in the Liver Are Generated <i>In Situ</i> from Their Own Preexisting Precursors
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- Shimizu Takao
- Department of Immunology, Niigagta University School of Medicine
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- Sugahara Satoshi
- Department of Immunology, Niigagta University School of Medicine
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- Oya Hiroshi
- Department of Immunology, Niigagta University School of Medicine
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- Maruyama Satoshi
- First Department of Surgery, Niigagta University School of Medicine
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- Minagawa Masahiro
- First Department of Surgery, Niigagta University School of Medicine
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- Bannai Makoto
- Department of Immunology, Niigagta University School of Medicine
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- Hatakeyama Katsuyoshi
- First Department of Surgery, Niigagta University School of Medicine
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- Abo Toru
- Department of Immunology, Niigagta University School of Medicine
抄録
Parabiotic pairs of B6.Ly5.1 and B6.Ly5.2 mice were used to investigate how lymphocytes in various organs and various lymphocyte subsets mixed with partner cells. The origin of partner cells was determined by using anti-Ly5.1mAb in conjunction with immunofluorescence tests. Parabiosis was also produced after the irradiation of B6.Ly5.2 mice at various doses to prepare an immunosuppressive partner. Irrespective of irradiation, lymphocytes and other hematopoietic cells in the bone marrow and lymphocytes in the thymus showed a low mixture of partner cells in comparison with those of all other organs tested. On the other hand, lymphocytes in the blood, spleen, and lymph nodes became a half-and-half mixture of their own cells and partner cells by 14 days after parabiosis. Among lymphocyte subsets, intermediate CD3 cells (i.e., CD3int cells) and NKT cells (i.e., NK1.1+ subset of CD3intcells) in the liver also showed a low mixture of partner cells. The present results raise the possibility that lymphocytes in the bone marrow and thymus, and extrathymic T cells in the liver might be in situ generated from their own preexisting precursor cells. Another observation was that, after irradiation, partner cells showed accelerated mixture even if they showed a low mixture under non-irradiated conditions. However, only lymphocyte subsets with the same phenotype as those of preexisting cells entered the corresponding sites.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 43 (6), 595-608, 1999
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1573387452894572288
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- NII論文ID
- 130003484737
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles