Ribotyping of <i>Vibrio parahaemolyticus</i> Isolates Obtained from Food Poisoning Outbreaks in Taiwan

• Wong Hin-chung

  Department of Microbiology, Soochow University

• Ho Chia-Yun

  Department of Microbiology, Soochow University

• Kuo Li-Ping

  Food Microbiology Division, National Laboratories of Food and Drug

• Wang Tien-Kuei

  Bacteriology Division, National Institute of Preventive Medicine

• Lee Chi-Lung

  Bacteriology Division, National Institute of Preventive Medicine

• Shih Daniel Yang-Chih

  Food Microbiology Division, National Laboratories of Food and Drug

抄録

Vibrio parahaemolyticus is a prevalent food-borne pathogen in Taiwan, Japan and other Asian countries. This work presents a novel ribotyping method for the molecular epidemiological examination of this pathogen. Genomic DNA was fragmented by HindIII digestion and hybridized with cDNA probe for Escherichia coli 16S and 23S RNA genes. A total of 121 isolates obtained from outbreaks during 1992 and 1994 in Taiwan were characterized by this ribotyping method. Four to seventeen restricted fragments were visualized in these isolates. After hierarchical cluster analysis, these isolates were grouped into thirty different ribotypes. In addition, A3, A7, E3 and F1 were the major ribotypes, consisting of 22.3, 13.2, 9.1, and 8.3% of the isolates, respectively. A, E, F, G and B were the major groups, consisting of 46.2, 14.0, 9.1, 6.7, and 6.7% of the isolates, respectively. The discriminatory ability of this ribotyping method, as determined by Simpson's index of diversity, was 0.93, which closely resembled that of a previously reported pulsed-field gel electrophoresis method.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 43 (7), 631-636, 1999

  Center For Academic Publications Japan

詳細情報

• CRID: 1570291228150758400
• NII論文ID: 130003484741
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00086374316?from=CiNii
• 本文言語コード: en
• データソース種別:
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