Phenotypic and Genotypic Homogeneity of the Strains of <i>Rickettsia japonica</i> Isolated from Patients with Oriental Spotted Fever

  • Uchiyama Tsuneo
    Department of Virology, School of Medicine, The University of Tokushima

抄録

Nine pathogenic strains of Rickettsia japonica isolated from patients with Oriental spotted fever were compared phenotypically and genotypically. Constitution and antigenicity of the proteins demonstrated to be the same among strains. Polymerase chain reaction (PCR) amplification of the two major outer membrane protein genes (ompA and ompB) and an intracellular spotted fever group-common antigen protein gene (rps120) produced the same sizes of products for all strains. Restriction fragment length polymorphism of the PCR products showed the same pattern among strains with each endonuclease. Thus, these strains belong to a single type, the same as the type strain YH (=ATCC VR-1363).

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