Nucleotide Sequencing and Transcriptional Analysis of Two Tandem Genes Encoding Glucosyltransferase (Water-Soluble-Glucan Synthetase) in <i>Streptococcus cricetus</i> HS-6
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- Inoue Miho
- Department of Microbiology, Okayama University Dental School
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- Inoue Tetsuyoshi
- Department of Microbiology, Okayama University Dental School
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- Miyagi Atsushi
- Department of Microbiology, Okayama University Dental School
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- Tanimoto Ichiro
- Department of Microbiology, Okayama University Dental School
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- Shingaki Ryuji
- Department of Microbiology, Okayama University Dental School
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- Ohta Hiroyuki
- Department of Microbiology, Okayama University Dental School
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- Fukui Kazuhiro
- Department of Microbiology, Okayama University Dental School
抄録
Two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (GTF-S) were cloned from the lambda gene library of Streptococcus cricetus HS-6 (serotype a) using anti-GTF-S antibody, and the nucleotide sequences were analyzed. The two genes (ORF1 and ORF2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and C-terminal repeated units for dextran binding, and [2] a homology search revealed that the ORF1 and ORF2 products are homologous to the GtfS protein (77.4%) of S. downei Mfe28 and GtfT protein (83.8%) of S. sobrinus OMZ176, respectively, which are both known to have GTF-S activity. Therefore, ORF1 and ORF2 might be designated gtfS and gtfT of S. cricetus, respectively. A Northern blotting and RNase protection assay suggested that the gtfS and gtfT of S. cricetus are transcribed as a single bicistronic mRNA as well as separate monocistronic mRNAs. Primer extension analysis indicated multiple transcriptional start points for each gene.
収録刊行物
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- MICROBIOLOGY and IMMUNOLOGY
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MICROBIOLOGY and IMMUNOLOGY 44 (9), 755-764, 2000
Center For Academic Publications Japan
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詳細情報 詳細情報について
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- CRID
- 1572543027964780672
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- NII論文ID
- 130003484903
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- ISSN
- 03855600
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles