Localization of Major, High Molecular Weight Proteins in <i>Bacteroides forsythus</i>

• Higuchi Naoya

  Department of Endodontics School of Dentistry, Aichi-Gakuin University

• Murakami Yukitaka

  Department of Microbiology School of Dentistry, Aichi-Gakuin University

• Moriguchi Keiichi

  Department of Anatomy, School of Dentistry, Aichi-Gakuin University

• Ohno Norikazu

  Department of Anatomy, School of Dentistry, Aichi-Gakuin University

• Nakamura Hiroshi

  Department of Endodontics School of Dentistry, Aichi-Gakuin University

• Yoshimura Fuminobu

  Department of Microbiology School of Dentistry, Aichi-Gakuin University

抄録

Bacteroides forsythus produces species-specific major proteins with high molecular weights of 270 and 230-kDa (270K and 230K). A specific antibody raised against 270K was used for Western blot analysis and immunoelectron microscopy. Western blot analysis showed that the 270K and 230K proteins were immunologically similar. Immunogold labeling of ultrathin-sectioned bacterial cells and biochemical fractionation revealed that these proteins were localized at the outermost cell surface, not in the cytoplasm. These results suggest that major proteins ubiquitous to this species may form the S-layer.

収録刊行物

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 44 (9), 777-780, 2000

  Center For Academic Publications Japan

詳細情報

• CRID: 1572824502941495552
• NII論文ID: 130003484906
• ISSN: 03855600
• Web Site: https://jlc.jst.go.jp/DN/JALC/00089025727?from=CiNii
• 本文言語コード: en
• データソース種別:
  • CiNii Articles