Immunohistological detection of B lymphocytes in the rat lymphoid organs by using anti-rat B lymphocyte serum.
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- YAMANAKA NOBORU
- Department of Pathology, Sapporo Medical College Department of Otolaryngology, Sapporo Medical College
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- ISHII YOSHIFUMI
- Department of Pathology, Sapporo Medical College
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- KOSHIBA HIROFUMI
- Department of Pathology, Sapporo Medical College
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- KIKUCHI KOKICHI
- Department of Pathology, Sapporo Medical College
書誌事項
- タイトル別名
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- Immunohistological Detection of B Lymph
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抄録
YAMANAKA, N., ISHII, Y., KOSHIBA, H. and KIKUCHI, K. Immunohistological Detection of B Lymphocytes in the Rat Lymphoid Organs by Using Anti-Rat B Lymphocytes Serum. Tohoku J. exp. Med., 1981, 135 (2), 187-196-Anti-rat B lymphocyte serum (ARBS) was prepared by immunizing rabbits with purified B cells from rat spleen and by absorbing the rabbit serum with rat red blood cells and syngeneic sarcoma cells. The specificity of ARBS for rat B cells was confirmed by various cytologic and immunofluorescence studies. By indirect immunofluorescence on tissue sections of rat spleen and lymph nodes, anatomical localizations of B cells in lymphoid organs were lymphoid follicles of the lymph node and lymphocyte corona surrounding periarteriolar lymphoid sheaths of the spleen; while cells located in the thymus-dependent area were essentially devoid of immunofluorescence or no cytoplasmic immunofluorescence was observed in plasma cells. Thus, the B cell distribution in tissue sections was clearly demonstrated with ARBS. When the cross-reactivity of ARBS with mouse B cells was studied by complement-dependent cytotoxicity and immunofluorescence, it was found that mouse B cells shared at least one determinant of rat B lymphocyte specific antigens (RBLA) with rat B cells, and that the distribution pattern of the cross-reacting antigens in mouse lymphoid tissues was essentially the same as that of RBLA in rat lymphoid organs.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 135 (2), 187-196, 1981
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390282679216177024
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- NII論文ID
- 130003492869
- 40005306830
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- NII書誌ID
- AA00863920
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- COI
- 1:STN:280:Bi2C38bovVw%3D
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- ISSN
- 13493329
- 00408727
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- NDL書誌ID
- 2360836
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- PubMed
- 7036408
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可