Two affinity immunoelectrophoretic methods for studying collagen interaction with von Willebrand factor antigen.
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- AIHARA MORIO
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- KUDO IKUO
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- ISHIGAKI HIROSHI
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- UENO KAORU
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- SAWADA YOSHIHIKO
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- YOSHIDA YUTAKA
- The First Department of Internal Medicine, Hirosaki University School of Medicine
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- COOPER HERBERT A.
- Department of Pathology and Center for Thrombosis and Hemostasis, the University of North Carolina, Chapel Hill
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- WAGNER ROBERT H.
- Department of Pathology and Center for Thrombosis and Hemostasis, the University of North Carolina, Chapel Hill
抄録
AIHARA, M., KUDO, I., ISHIGAKI, H., UENO, K., SAWADA, Y., YOSHIDA, Y., COOPER, H.A. & WAGNER, R.H. Two Affinity Immunoelectrophoretic Methods for Studying Collagen Interaction with von Willebrand Factor Antigen. Tohoku J. exp. Med., 1987, 153 (2), 169-177-Two new immunoelectrophoretic methods are described for studying the interaction of collagen fibrils with von Willebrand factor antigen (vWF:Ag). In the first, the sample was electrophoresed through a collagen-agarose wedge into an antibody-agarose area, and immunoprecipitin lines was detected by staining. Different immunoprecipitin patterns were obtained with the vWF:Ag of normal plasma, commercial FVIII preparations, and von Willebrand disease (vWD) type ha plasma as the result of collagen binding of vWF:Ag. In the other method, the sample was electrophoresed into agarose for preliminary separation of forms, followed by migration in the second dimension through a collagen spacer gel into an antibody-agarose area. This method demonstrated preferential binding of high molecular weight forms of vWF:Ag in normal plasma and slight binding of the lower molecular weight forms of antigen found in vWD type ha plasma. The affinity wedge method is a convenient general method for finding quickly a useful concentration of affinity reagent.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 153 (2), 169-177, 1987
東北ジャーナル刊行会
