Endothelial cells create a hematopoietic inductive microenvironment preferential to erythropoiesis in the mouse spleen.
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- Yanai Nobuaki
- Department of Cell Biology, Research Institute for Tuberculosis and Cancer, Tohoku University
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- Satoh Takeshi
- Department of Cell Biology, Research Institute for Tuberculosis and Cancer, Tohoku University
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- Obinata Masuo
- Department of Cell Biology, Research Institute for Tuberculosis and Cancer, Tohoku University
抄録
The spleen is an erythropoietic organ in mouse. To reconstruct a microenvironment essential for erythropoiesis in vitro, the stroma (MSS31) cell line had been established from newborn mouse spleens. MSS31 cells exhibited properties of endothelial cells: (a) the cells showed the activity to uptake acetylated low-density lipoprotein (Ac-LDL) and (b) the cells can form a capillarylike structure by a phenotypic modulation in collagen matrices. MSS31 cells selectively supported the proliferation and differentiation of the erythroid progenitor cells by direct cell-to-cell contact in a semisolid medium in the presence of erythropoietin. These layers also supported erythrocyte maturation and enucleation of erythroblasts. This suggests that spleen endothelial cells are a new type of stromal cell with erythropoietic stimulation activity and may have a critical function in the hemopoietic inductive microenvironment of the mouse spleen.
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 16 (1), 87-93, 1991
日本細胞生物学会
