Effects of Sulphydryl Reagents on Receptor-Mediated Hormonal Responses at the Cellular Level: Insulin-mimicking Characteristics of Thiol-blocking Compounds in Rat Hepatocyte Primary Culture.
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- H. Boot J.
- Department Veterinary Pharmacology, Pharmacy and Toxicology
抄録
The interference of various SH-blocking chemicals with the insulin-controlled regulation of the hepatic carbohydrate metabolism was studied in rat hepatocyte primary cultures. The organic mercurials PCMB, PCMBS, mersalyl the disulphide agents DTP, CPDS, disulfiram and the SH-alkylating reagent NEM were used as experimental SH-blocking model compounds. All studied compounds, except for NEM, induced an increased glycogen deposition comparable with the physiological insulin-induced glycogen-deposition. PCMBS appeared to be the most effective insulin-mimicking anabolic trigger. The action of the insulin molecule itself was potentiated by PCMBS as well as demonstrated by increased glycogen deposition, induced pyruvate kinase (PK) and decreased phosphoenol-pyrnvate carboxykinase (PEP-CK) activity. However, cell-exposure to insulin and PCMBS in relatively high doses was destructive, as demonstrated by decreased glycogen levels, most probably as a result of insulin-receptor overstimulation and metabolic stress. Thus, SH-blocking compounds are able to trigger insulin-dependent metabolic processes. The relatively non-permeant organic mercurial PCMBS proved to be the most effective insulin-mimicking SH-blocking compound.
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 21 (1), 1-6, 1996
日本細胞生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204697194368
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- NII論文ID
- 130003512926
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- DOI
- 10.1247/csf.21.1
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- COI
- 1:CAS:528:DyaK28XivVKjurw%3D
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- ISSN
- 13473700
- 03867196
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- PubMed
- 8726468
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可