Metabolism of 1-aminocyclopropane-1-carboxylic acid.

• HONMA Mamoru

  Department of Agricultural Chemistry, Hokkaido University

• SHIMOMURA Tokuji

  Department of Agricultural Chemistry, Hokkaido University

抄録

Several microorganisms capable of utilizing 1-aminocyclopropane-l-carboxylate (ACPC) were isolated from soil. A bacterium which belongs to Pseudomonas accumulated cellular a-aminobutyrate with consumption of ACPC and cells incubated with ACPC medium had the activity deaminating the substrate to form α-ketobutyrate. An enzyme, ACPC deaminase, was highly purified and its molecular weight, substrate specificity and absorption spectrum were investigated. These results suggested that this enzyme was a pyridoxal 5'-phosphate enzyme which has the molecular weight of 104000 and high specificity for ACPC, Km=1.5mM. A yeast, Hansenula saturnus, is also capable of forming ACPC deaminase, which has a lower molecular weight, 69000, and higher Km value, 2.6mM.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 42 (10), 1825-1831, 1978

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206468574336
• NII論文ID: 130003525829
• DOI: 10.1271/bbb1961.42.1825
• COI: 1:CAS:528:DyaE1MXhtFKru7k%3D
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可