Preliminary Studies on Chemiluminescence Immunoassay for Human Chorionic Gonadotropin and Thyrotropin Using Acridinium Ester-Labeled Antibody.
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- SATO Naofumi
- Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.
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- SHIRAKAWA Kamon
- Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.
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- KAKIHARA Youko
- Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.
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- MOCHIZUKI Hiroshi
- Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.
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- KANAMORI Tosinori
- Biosciences Research Laboratory, Mochida Pharmaceutical Co. Ltd.
書誌事項
- タイトル別名
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- Preliminary Studies on Chemiluminescenc
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A highly sensitive chemiluminescence assay system for human chorionic gonadotropin (hCG) and thyroid stimulating hormone (TSH) was developed using a newly synthesized acridinium ester (AE) with a binding substitutional group at the N-10 position and having excellent chemiluminescence and stability. AE-labeled antibodies with an excellent chemiluminescence intensity were prepared after studying the labeling of anti-hCG antibodies with AE under different conditions. By studying the purification conditions for AE-labeled antibodies we produced AE-labeled antibodies having a low background value when assayed. When the detection limit is defined as the signal value+3SD at a concentration of zero for each substance measured, the detection limit for hCG is 0.01IU/l and that for TSH is 0.001μIU/ ml, demonstrating extremely high sensitivity. The labeled antibody′s stability is also excellent.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 12 (6), 853-858, 1996
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204255700480
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- NII論文ID
- 130003528107
- 10002409879
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK28XnsFChtb4%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4099078
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4099078
- https://link.springer.com/content/pdf/10.2116/analsci.12.853.pdf
- https://link.springer.com/article/10.2116/analsci.12.853/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci1985/12/6/12_6_853/_pdf
- https://search.jamas.or.jp/link/ui/1997104004
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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