Determination of carboxylesters by indirect photometric ion chromatography with an on-line enzyme immobilized column.

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Carboxylesters, carboxylic and inorganic anions were determined separately by photometric ion chromatography with an enzyme immobilized column. In the system, a carboxylesterase immobilized column (4.6mm i.d.×10mm), which converted carboxylesters to carboxylic anions completely, was set between a sample injector and an analytical column. The other chromatographic conditions were as follows: an anion exchanger-type separation column (4.6mm i.d.×250mm); phthalate-N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid e uent (pH 6.5); an oven of temperature 40°C; a flow rate of 1.0ml/min; 280-nm UV detection; and an injection volume of 20μl. Calibration curves for ethyl esters of acetate, propionate, butyrate and isova erate were linear with the range 5×10-10mol to 1×10-7mol according to the peak-height method; their detection limits were 10-10 mol levels. The present method was applied for the determination of carboxylesters in practical samples.

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  • Analytical Sciences

    Analytical Sciences 5 (6), 691-695, 1989

    社団法人 日本分析化学会

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