A calculation method of correspoding mobile phase composition for obtaining the same retention with different binary organic/aqueous solvent systems in reversed phase liquid chromatography.

• OKAMURA Kazuyo M.

  JASCO, Japan Spectroscopic Co., Ltd.

• HIBI Kiyokatsu

  JASCO, Japan Spectroscopic Co., Ltd.

• NAKAMURA Hiroshi

  Department of Analytical Faculty of Pharmaceutical Sciences, The University of Tokyo

抄録

A practical calculation method of organic solvent composition was proposed for obtaining the same retention with different binary solvent systems in reversed phase liquid chromatography. It was shown that there is a good correlation between log k′ of each test solute and the solubility parameter δ′ of each solvent mixture calculated from the Hildebrand solubility parameter δ of each pure solvent. We established a calculation method of corresponding mobile phase composition for obtaining the same retentions, by simply inserting the solubility parameters of water and an organic solvent of interest into the equation used for δ′ calculation.

収録刊行物

• Analytical Sciences

  Analytical Sciences 6 (6), 857-861, 1990

  社団法人 日本分析化学会

キーワード

• Reversed phase liquid chromatography
• calculation method
• capacity factor
• organic solvent composition
• retention behavior
• Hildebrand solubility parameter

詳細情報

• CRID: 1390001204253307776
• NII論文ID: 130003529044
• DOI: 10.2116/analsci.6.857
• COI: 1:CAS:528:DyaK3MXnsVCqsQ%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.6.857.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.6.857/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/6/6/6_6_857/_pdf
• 本文言語コード: en
• データソース種別:
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