Application of Supercritical Fluid Extraction and Chromatography to Assay Fat-Soluble Vitamins in Hydrophobic Ointment.

• MASUDA Mitsuhiro

  Research Center, Taisho Pharmaceutical Co., Ltd.

• KOIKE Shinichi

  Research Center, Taisho Pharmaceutical Co., Ltd.

• HANDA Mitsuichi

  Research Center, Taisho Pharmaceutical Co., Ltd.

• SAGARA Kazuhiko

  Research Center, Taisho Pharmaceutical Co., Ltd.

• MIZUTANI Taku

  Research Center, Taisho Pharmaceutical Co., Ltd.

抄録

The determination of retinol palmitate and tocopherol acetate in a hydrophobic ointment by a coupled supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) system is described. The ointment, triturated with diatomaceous earth powder, was subjected to SFE with carbon dioxide, and the extract containing fat-soluble vitamins was trapped on a trimethylsilyl silica-gel column by reducing the fluid pressure. The trapped components were analyzed on an octadecylsilyl silica-gel column by SFC using carbon dioxide containing ethanol as a mobile phase and UV absorption monitoring at 284nm. In this method, retinol palmitate and tocopherol acetate in preparations could be determined within ten minutes without any manual products after placing the sample into the system.

収録刊行物

• Analytical Sciences

  Analytical Sciences 9 (1), 29-32, 1993

  社団法人 日本分析化学会

キーワード

• Supercritical fluid extraction
• supercritical fluid chromatography
• fat-soluble vitamin
• retinol palmitate
• tocopherol acetate

詳細情報

• CRID: 1390282679232883200
• NII論文ID: 130003529898
• DOI: 10.2116/analsci.9.29
• COI: 1:CAS:528:DyaK3sXhslGqsLY%3D
• ISSN: 13482246
• ISSN: 09106340
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.9.29.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.9.29/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/9/1/9_1_29/_pdf
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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