Cloning and Characterization of a cDNA Encoding a Novel Heterogeneous Nuclear Ribonucleoprotein-Like Protein and Its Expression in Myeloid Leukemia Cells.
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- Tsuchiya Naoto
- Graduate School of Integrated Science, Yokohama City University
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- Kamei Daisuke
- Graduate School of Integrated Science, Yokohama City University
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- Takano Akira
- Graduate School of Integrated Science, Yokohama City University
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- Matsui Takaaki
- Graduate School of Integrated Science, Yokohama City University
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- Yamada Michiyuki
- Graduate School of Integrated Science, Yokohama City University
抄録
We isolated a cDNA encoding a novel heterogeneous nuclear ribonucleoprotein (hnRNP)-like protein on DNA affinity screening of a K562 cDNA expression library with an oligodeoxynucleotide (JKT41) derived from intron 9 of the human myeloperoxidase gene. The cDNA has a 1, 305 by sequence that encodes a polypeptide of 301 amino acid residues. The protein, named JKTBP, contains two repeats of a putative RNA binding domain (RBD), each composed of canonical RNP-2 and RNP-1 motifs, and a glycine- and tyrosine-rich carboxyl terminus. The sequences of these two repeats are highly homologous with those of the 2×RBD-Gly rich group of hnRNPs. Northern blotting showed that two mRNAs of approximately 1.4 and 2.8 kb were present in most cultured cells examined. The recombinant protein expressed in Escherichia coli interacted with the double-stranded form of JKT41 as well as with its single-stranded form. This interaction was competitively inhibited by the same unlabeled JKT41 and to nearly the same extent by unrelated oligonucleotides. Moreover, the recombinant protein interacted with poly (G) and poly (A), but not with poly (U) or poly (C). Transient expression of the protein in SKM-1 cells repressed the expression of chloramphenicol acetyltransferase reporter genes located downstream of the intron 9 element of JKT41 or intron 7 element of FERE27. The implications of the protein in the biogenesis of mRNA are discussed.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 123 (3), 499-507, 1998
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1573105977964090624
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- NII論文ID
- 130003533389
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles