Study on the Biosynthesis of Dolichol in Yeast. Recognition of the Prenyl Chain Length in Polyprenol Reduction.

• Tateyama Seiji

  Institute Chemical Reaction Science Tohoku University

• Sagami Hiroshi

  Institute Chemical Reaction Science Tohoku University

抄録

We synthesized three water-soluble biotin-tagged compounds with different prenyl chain lengths, biotinylated farnesal (BF), biotinylated C₅₅ polyprenal (BP55), and biotinylated C₈₀-polyprenol (BP80), and examined their effects on in vitro dolichol synthesis from farnesyl diphosphate. BF and BP55 did not affect the dolichol synthesis, whereas BP80 inhibited the reduction pathway from polyprenol to dolichol, accompanied by a decrease in the entire polyprenol and dolichol synthesis. Comparison of BP80 with eighteen detergents, including Triton X-100, CHAPS, octylglucoside, deoxycholate, and Tween 80, revealed the specific effect of BP80 on the reduction pathway. On SDS-polyacrylamide gel electrophoresis, BP80 was detected in an associated form with a 50 kDa protein. These results suggest that the reduction of polyprenol to dolichol in the dolichol biosynthetic pathway proceeds with the recognition of the polyprenol chain length by a 50 kDa protein.

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 129 (2), 297-302, 2001

  The Japanese Biochemical Society

キーワード

• dolichol
• <i>N</i>-linked glycoprotein biosynthesis
• polyprenol
• polyprenol reduction
• reductase

詳細情報

• CRID: 1570291228197148160
• NII論文ID: 130003534084
• ISSN: 0021924X
• Web Site: https://jlc.jst.go.jp/DN/JALC/00090342455?from=CiNii
• 本文言語コード: en
• データソース種別:
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