Purification of Tyrosine: tRNA Ligase from <I>Saccharomyces cerevisiae</I> αS288C
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- KUCAN Zeljko
- Department of Biochemistry, New York University School of Medicine
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- CHAMBERS Robert W.
- Department of Biochemistry, New York University School of Medicine
抄録
L-Tyrosine: tRNA ligase (AMP)[EC 6. 1. 1. 1] has been purifeed te homogeneity from a genetically well-characterized, haploid strain of yeast, Saccharomyces cerevisiae α S288C. The kinetic constants for tRNATyr are: Km=3.5×10-7; kcat=365 min-1. Under dissociating conditions, the enzyme gives a single band on discontinuous gel electrophoresis, corresponding to a molecular weight=31, 500. Under non-dissociating conditions, where the activity of the enzyme is retained, the molecular weight estimated by gel filtration is 116, 000. Though tentative, these data indicate this enzyme may be composed of four subunits.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 73 (4), 811-819, 1973
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1571417128103319808
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- NII論文ID
- 130003539019
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles