Effects of polyamines on the degradation of ribonucleic acids by polynucleotide phosphorylase of Micrococcus luteus.
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- IGARASHI Kazuei
- Faculty of Pharmaceutical Sciences, Chiba University
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- KUMAGAI Hiroshi
- Faculty of Pharmaceutical Sciences, Chiba University
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- OGUCHI Hirofumi
- Faculty of Pharmaceutical Sciences, Chiba University
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- HIROSE Seiyu
- Faculty of Pharmaceutical Sciences, Chiba University
抄録
The effects of polyamines on the breakdown of synthetic polynucleotides [poly(A), poly(C), and poly(U) ] by polynucleotide phosphorylase [polyribonucleotide: orthophosphate nucleotidyltransferase, EC 2. 7. 7. 8] from Micrococcus luteus have been studied. Although the breakdown of all the synthetic polynucleotides tested was stimulated by polyamines, the degree of stimulation by polyamines was in the order poly(C)>poly(A)>poly(U) at pH 7.5. However, the difference in degree of stimulation among polynucleotides decreased as the pH or monovalent cation concentration was increased. In the presence of heparin, an inhibitor of polynucleotide phosphorylase hydrolysis of polynucleotides, spermidine clearly stimulated the breakdown of poly(C) and poly(A), while the breakdown of poly(U) was stimulated only slightly by the addition of spermidine. Although binding of [14C]spermine to polynucleotide phosphorylase was observed by gel filtration, the amount of spermine bound to the enzyme was much less than that to RNA.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 81 (2), 389-394, 1977
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1570572703134072064
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- NII論文ID
- 130003540137
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles