Re-examination of the ColE1 DNA regions essential for autonomous replication and their functions.
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- KIMURA Akinori
- Department of Biochemistry, Kyushu University 60, School of Medicine
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- SHIMADA Kazunori
- Department of Biochemistry, Kyushu University 60, School of Medicine
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- TAKAGI Yasuyuki
- Department of Biochemistry, Kyushu University 60, School of Medicine
抄録
Starting from pAO3, a plasmid consisting of a quarter of colicinogenic factor E1 (ColE1) DNA, various small ColE1 derivatives were constructed by in vitro recombination and their ability to achieve autonomous replication was examined. The 436 base pair HaeIII-C fragment of pAO3 contained information for replication when it was recombined with the non-replicating Amp fragment. However, when it was connected to other DNA fragments, the resulting hybrid molecules were not isolated as plasmids. The present results indicate that the additional region of about 240 base pairs next to the HaeIII-C fragment of ColE1 is also essential for the maintenance of a plasmid state. <br>Moreover, using various small ColE1 derivatives, the DNA region responsible for the interference and incompatibility functions of ColE1 DNAs was located. The results indicate that the interference and incompatibility functions are coded by the same ColE1 DNA segment and are not essential for the maintenance of a plasmid state.
収録刊行物
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- The Journal of Biochemistry
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The Journal of Biochemistry 92 (1), 95-103, 1982
The Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1571135653087137280
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- NII論文ID
- 130003542243
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- ISSN
- 0021924X
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- 本文言語コード
- en
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- データソース種別
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- CiNii Articles