Adsorption chromatography of nucleic acids on silicone-coated porous glass.

• MIZUTANI Takaharu

  Faculty of Pharmaceutical Sciences, Nagoya City University

抄録

Bovine liver tRNA was adsorbed on silicone-coated porous glass in 5M NaCl, 10mM Tris-HCI (pH 7.6) and fractionated by elution with decreasing NaCI concentrations. tRNA^Pro, tRNA^Val, tRNA^Ile, tRNA^Thr, ttRNA^Ser, and tRNA^Phe were eluted in this order. tRNA which had been digested with ribonuclease A was not adsorbed. Qβ RNA (adsorbed onto the glass in 5M NaCl) was eluted with 1.5M NaCl. RNA species in a crude rRNA fraction from Escherichia coli were separated into tRNA, 5S rRNA, and high molecular weight rRNA on siliconized porous glass. A half of calf thymus DNA was adsorbed on the glass in 5M NaCl and the residual part passed through the column. The CD spectra showed that DNA and tRNA took the C-form and the A-form in 5M NaCl, respectively. Therefore, the discrepancies of behavior of the DNA and RNA on siliconized porous glass may be related to the occurrence of these forms. The recovery of these nucleic acids from the column was 83-100%. Adsorption chromatography on siliconized porous glass may be a useful method for the separation of tRNA, rRNA, and mRNA.

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 94 (1), 163-169, 1983

  The Japanese Biochemical Society

詳細情報

• CRID: 1572261552994744576
• NII論文ID: 130003542536
• ISSN: 0021924X
• Web Site: https://jlc.jst.go.jp/DN/JALC/00169151872?from=CiNii
• 本文言語コード: en
• データソース種別:
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