界面活性物質を供試した臓器材料からのPCR用マイコプラズマDNAテンペレート簡易作製法

• 小林 秀樹

• Munthali Gift

• 宮本 千加子

• 両角 徹雄

• 三谷 賢冶

• 伊東 伸宣

• 塩野 浩紀

• 山本 孝史

書誌事項

タイトル別名

• A Simple Preparation of Mycoplasmal DNA Template for PCR from Biological Samples Using Effective Surfactants.
• simple preparation of mycoplasmal DNA t

抄録

To prepare mycoplasmal DNA template for PCR from biological samples rapidly and easily, surfactants which can solubilize cell membrane effectively were investigated. 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) was considered an effective surfactant. This surfactant could solubilize mycoplasma cell membrane without suppressing the polymerase reaction. In addition, proteinase K treatment played an important role in preparing mycoplasmal DNA template from a simulated biological sample. It was therefore considered that a combination of proteinase K- and CHAPS- added lysis buffer would be more useful in preparing mycoplasmal DNA template. We could detect PCR products by using the lysis buffer with a simulated lung emulsion sample containing mycoplasma organisms at 10⁴ CFU per gram.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 58 (5), 477-479, 1996

  公益社団法人 日本獣医学会

キーワード

• DNA template
• mycoplasma
• surfactant

詳細情報

• CRID: 1390282681401876480
• NII論文ID: 130003572404
• NII論文ID: 110003916540
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.58.477
• COI: 1:CAS:528:DyaK28Xktlyksbs%3D
• ISSN: 13477439
• ISSN: 09167250
• ISSN: http://id.crossref.org/issn/09167250
• NDL書誌ID: 3960568
• PubMed: 8741614
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3960568
• Web Site: http://www.jstage.jst.go.jp/article/jvms1991/58/5/58_5_477/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996209492
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可