Introduction of cytokine genes into rat solid neoplasms with a hand-held gene gun

• Hosoya Yoshinori

  Departments of Clinical Pharmacology, Jichi Medical School Departments of Surgery, Jichi Medical School

• Kobayashi Eiji

  Departments of Clinical Pharmacology, Jichi Medical School

• Yoshida Yoko

  Departments of Biochemistry, Jichi Medical School

• Tomizawa Naoko

  Departments of Clinical Pharmacology, Jichi Medical School

• Fujimura Akio

  Departments of Clinical Pharmacology, Jichi Medical School

• Kagawa Yasuo

  Departments of Biochemistry, Jichi Medical School

• Kanazawa Kyotaro

  Departments of Surgery, Jichi Medical School

書誌事項

タイトル別名

• I. Effect of IL-12 plus TNF-α gene transfection on subcutaneous tumor

抄録

Using a hand-held gene gun which accelerates DNA-coated microparticles, we examined the effect of interleukin-12 (IL-12) and/or tumor necrosis factor-α (TNF-α) gene transfection a rat solid tumor model. Yoshida sarcoma cells were inoculated subcutaneously into the chests of Donryu rats. Then 4μg of IL-12 and/or TNF-α DNA-coated gold (Au) particles was injected into the tumor by pressurized helium (He) gas at 400psi (1psi=6, 890Pa) on day 5 and 7 after tumor inoculation. The corresponding dose of β-galactosidase was given as a control. Compared with the control, injection of TNF-α, IL-12, and IL-12 plus TNF-α effectively inhibited tumor growth and improved the survival rate. TNF-α alone prolonged the survival of tumor-bearing rats, but the animals still died within 41 days after tumor injection, On the other hand, IL-12 alone and IL-12 plus TNF-α completely cured 20% and 40% of the rats, respectively. IL-12 plus TNF-α gene therapy using the gene gun showed a synergetic effect on the survival of tumor-bearing rats. This procedure might useful for the clinical treatment of solid cancer. In particular, there is a possibility of intraoperative gene transfection because the gene gun can introduce multiple genes very rapidly.

収録刊行物

• Ａｎｎａｌｓ　ｏｆ　Ｃａｎｃｅｒ　Ｒｅｓｅａｒｃｈ　ａｎｄ　Ｔｈｅｒａｐｙ

  Ａｎｎａｌｓ　ｏｆ　Ｃａｎｃｅｒ　Ｒｅｓｅａｒｃｈ　ａｎｄ　Ｔｈｅｒａｐｙ 7 (1), 39-42, 1998

  日本癌病態治療研究会

キーワード

• <i>gene therapy</i>
• <i>gene gun</i>
• <i>solid tumor</i>
• <i>interleukin-12</i>
• <i>tumor necrosis factor-α</i>

詳細情報

• CRID: 1390001205190741120
• NII論文ID: 130003607561
• DOI: 10.4993/acrt1992.7.39
• ISSN: 18805469
• ISSN: 13446835
• Web Site: https://www.jstage.jst.go.jp/article/acrt1992/7/1/7_1_39/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可