A Simple Solid-Phase Radioimmunoassay for Glutamic Acid Decarboxylase(GAD) Antibodies in Patients with Diabetes Mellitus.

• OHTA Mitsuhiro

  Clinical Research Center, Utano National Hospital

• OBAYASHI Hiroshi

  Kyoto Microbiological Institute

• TAKAHASHI Kenji

  Department of Medicine, Kurashiki Central Hospital

• KITAGAWA Yoshihiro

  First Department of Medicine, Kyoto Prefectural University of Medicine

• NAKANO Koji

  First Department of Medicine, Kyoto Prefectural University of Medicine

• MATSUO Satoshi

  Department of Pediatrics, Kyoto Prefectural University of Medicine

• OHISHI Mariko

  Department of Medicine, National Kyoto Hospital

• ITOH Nobuyuki

  Department of Genetic Biochemistry, Faculty of Pharmaceutical Sciences, Kyoto University

• HAYASHI Kyozo

  Department of Pharmaceutics, Gifu Pharmaceutical University

• OHTA Kiyoe

  Clinical Research Center, Utano National Hospital

抄録

Antibodies to glutamic acid decarboxylase (GAD Ab's) provide an important serologic marker for insulin-dependent diabetes mellitus (IDDM), but the diagnostic sensitivity and specificity of GAD antibodies have yet to be defined in assay systems suitable for screening a large number of samples. Therefore, using affinity purified GAD from rat brain we have developed a simple and sensitive solid-phase radioimmunoassay for measuring antibodies against the isoforms GAD₆₅ and GAD₆₇ and have examined a large number of serum samples from Japanese patients with IDDM of short-duration (less than 3 years) as well as from those with non-insulin-dependent diabetes mellitus (NIDDM). We detected GAD Ab in 62% (37 of 60) of the patients with IDDM, but only in 8% (4 of 51) of those with NIDDM. Almost all the patients with other autoimmune diseases and the healthy controls had no GAD Ab at all. Seventy-eight percent of the IDDM patients with GAD Ab concomitantly had islet cell cytoplasmic antibody (ICA), and there was a significant correlation between both autoantibody levels. This assay provides for rapid and simple screening for the early diagnosis of IDDM.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 20 (2), 139-148, 1996

  一般社団法人　日本酸化ストレス学会

キーワード

• Clinical Biochemistry
• Nutrition and Dietetics
• Medicine (miscellaneous)

詳細情報

• CRID: 1390282679648805504
• NII論文ID: 130003670388
• DOI: 10.3164/jcbn.20.139
• ISSN: 18805086
• ISSN: 09120009
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可