Contribution of Xanthine Oxidase-Derived Oxygen Free Radicals to the Development of Carbon Tetrachloride-Induced Acute Liver Injury in Rats

• Ohta Yoshiji

  Department of Chemistry, School of Medicine, Fujita Health University

• Kongo-Nishimura Mutsumi

  Department of Pediatric Surgery, School of Medicine, Fujita Health University

• Imai Yoichiro

  Department of Clinical Chemistry, Fujita Health University College

• Kishikawa Teruaki

  Department of Pediatric Surgery, School of Medicine, Fujita Health University

抄録

We examined how oxygen free radicals derived from xanthine oxidase (XOD) contribute to the development of carbon tetrachloride (CCl₄)-induced acute liver injury in rats. In rats treated with CCl₄ (1ml/kg), liver injury appeared 3h after the treatment and developed by 24h, judged from the serum levels of transaminases. Hepatic and serum XOD activities and serum uric acid concentration did not change 6h after CCl₄ treatment, but they all increased at 12h and further increased at 24h. An increase in the concentration of thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation, and decreases in reduced glutathione concentration and Cu, Zn-superoxide dismutase (SOD) activity, but not Mn-SOD activity, were observed in the liver at 6h after CCl₄ treatment and these changes were further enhanced at 24h. When allopurinol (50mg/kg), an XOD inhibitor, was administered to CCl₄-treated rats 6h after CCl₄ treatment, liver injury progression was prevented with attenuation of increased hepatic and serum XOD activities, serum uric acid concentration, and hepatic TBARS concentration and decreased hepatic reduced glutathione concentration and Cu, Zn-SOD activity at 24h after CCl₄ treatment. These results indicate that XOD-derived oxygen free radicals contribute to the progression of CCl₄-induced acute liver injury in rats both by stimulating hepatic lipid peroxidation and by disrupting hepatic antioxidant defense system.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 33 (3), 83-93, 2003

  一般社団法人　日本酸化ストレス学会

キーワード

• liver injury (rat)
• carbon tetrachloride
• xanthine oxidase
• oxygen free radicals
• allopurinol

詳細情報

• CRID: 1390282679649008512
• NII論文ID: 130003670583
• DOI: 10.3164/jcbn.33.83
• COI: 1:CAS:528:DC%2BD2cXjtFGkt7c%3D
• ISSN: 18805086
• ISSN: 09120009
• ISSN: http://id.crossref.org/issn/09120009
• Web Site: https://search.jamas.or.jp/link/ui/2005139540
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可