Comparison of the Biochemical and Immunological Properties of Nerve Growth Factors from Various Animals

• NAKATA Yasuhiro

  Department of Pharmaceutics, Gifu Pharmaceutical University

• NOMOTO Hiroshi

  Department of Pharmaceutics, Gifu Pharmaceutical University

• TAKAHASHI Ikuma

  Department of Pharmaceutics, Gifu Pharmaceutical University

• FURUKAWA Yoshiko

  National Center of Neurology and Psychiatry

• FURUKAWA Shoei

  National Center of Neurology and Psychiatry

• HAYASHI Kyozo

  Department of Pharmaceutics, Gifu Pharmaceutical University

抄録

Nerve growth factors (NGF's) were purified from mouse submaxillary gland, guinea pig prostate gland, bovine seminal plasma, and venom of Naja naja atra, and their biochemical and immunological properties were compared. The NGF's from the four sources stimulated the maximal response of nerve fiber outgrowth from chick dorsal root ganglia at 10ng/ml. Two-site enzyme immunoassay (EIA) systems for these NGF's showed that mouse NGF, guinea pig NGF, and bovine NGF were similar in immunological properties and that the crossreactivity was less than 1% between snake NGF and mammalian NGF's. However, pretreatment with anti-snake NGF antibody inhibited the biological activity of mammalian NGF's even when their immunological activity was not completely inhibited. These results suggest that snake NGF and mammalian NGF's are similar in their biological activity but not in their immunological activity and that anti-snake NGF antibody recognizes the parts of mammalian NGF's essential for the biological activity.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　Ｂｉｏｃｈｅｍｉｓｔｒｙ　ａｎｄ　Ｎｕｔｒｉｔｉｏｎ 4 (1), 73-86, 1988

  一般社団法人　日本酸化ストレス学会

キーワード

• nerve growth factor
• biochemical properties
• immunological properties
• mammalian NGF's
• snake NGF

詳細情報

• CRID: 1390282679649084288
• NII論文ID: 130003670609
• DOI: 10.3164/jcbn.4.73
• ISSN: 18805086
• ISSN: 09120009
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可