クリプトコックス症診断用ラテックス試薬の開発と評価

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  • Development and comparative evaluation of anti-Cryptococcus neoformans antibody coated latex reagent for serodiagnosis of cryptococcosis.

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Development of a system for detecting cryptococcal antigen in clinical specimens using latex particles sensitized with anti-C. neoformans globulin was attempted. We examined the optimal condition of the sensitized latex particle using anti-C. neoformans globulin fractionated by 40% saturated ammonium sulfate. Latex particles, with a diameter of 0.81μm, were sensitized with 20μg of anti-C. neoformans globulin per 1mg of latex. We compared the minimal concentration of detectable polysaccharide antigen by the sensitized latex particles and the commercially available particles and obtained almost the same sensitivity, but the former was also capable of detecting the antigen forming soluble immune complex in a patient's serum. The latex agglutination test was performed using a mixture of 75μl of protease treated serum or cerebropsinal fluid (CSF) and 25μl of sensitized latex suspension. After 10 minutes reaction on a rotator, the agglutination was read. The sensitivity of the latex agglutination test using the sensitized latex particles was found to be 100% in cases of cryptococcal meningitis, 81.8% in pulmonary cryptococcosis and 75% in cutaneous cryptococcosis. The specificity of the test was 100% with sera and 95% with CSF. It was concluded that the sensitized latex particles could be very useful in antigen detection for rapid and accurate serological diagnosis of cryptococcosis.

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