抗真菌薬はアトピー性皮膚炎患者Ｔ細胞のＩＬ‐４，ＩＬ‐５産生を抑制する

• 神田 奈緒子

  帝京大学医学部皮膚科

書誌事項

タイトル別名

• Antimycotics Suppress Interleukin-4 and Interleukin-5 Production in Anti-CD3 plus Anti-CD28-Stimulated T Cells from Patients with Atopic Dermatitis

抄録

It is reported that antimycotic agents are effective for the treatment of patients with atopic dermatitis (AD). We studied in vitro effects of antimycotics on T helper-1 and T helper-2 cytokine production in anti-CD3 plus anti-CD28-stimulated T cells from AD patients and normal donors. The amounts of interleukin-4 (IL-4) and IL-5 secreted by anti-CD3/CD28-stimulated T cells were higher in AD patients than in normal donors. Azole derivatives, ketoconazole, itraconazole, miconazole and non-azole terbinafine hydrochloride and tolnaftate reduced IL-4 and IL-5 secretion without altering that of IFN-γ and IL-2 in anti-CD3/CD28-stimulated T cells from both AD patients and normal donors. The azole derivatives were more inhibitory than non-azole antimycotics. These antimycotics reduced the anti-CD3/CD28-induced mRNA expression and promoter activities for IL-4 and IL-5. The cAMP analogue dibutyryl cAMP reversed the inhibitory effects of the antimycotics on IL-4 and IL-5 secretion, mRNA expression, and promoter activities. Anti-CD3/CD28 transiently (≤5min) increased intracellular cAMP in T cells, and the increase was greater in AD patients than in normal donors. The increase of cAMP by anti-CD3/CD28 correlated with IL-4 and IL-5 secretion by anti-CD3/CD28. The transient cAMP increase was suppressed by antimycotics, and azole derivatives were more suppressive than non-azoles. Azole derivatives inhibited the activity of cAMP-synthesizing adenylate cyclase while terbinafine hydrochloride and tolnaftate enhanced the activity of cAMP-hydrolyzing cyclic nucleotide phosphodiesterase in AD and normal T cells. These results suggest that the antimycotics may suppress IL-4 and IL-5 production by reducing cAMP signal, and strengthen the concept of their potential use for the suppression of T helper-2-mediated allergic reactions.

収録刊行物

• 日本医真菌学会雑誌

  日本医真菌学会雑誌 45 (3), 137-142, 2004

  日本医真菌学会

キーワード

• サイクリックAMP
• T helper-2細胞
• アトピー性皮膚炎
• 抗真菌薬

詳細情報

• CRID: 1390001204212743296
• NII論文ID: 130003672089
• NII論文ID: 10013334315
• NII書誌ID: AN10166867
• DOI: 10.3314/jjmm.45.137
• ISSN: 18820476
• ISSN: 09164804
• PubMed: 15284827
• Web Site: https://search.jamas.or.jp/link/ui/2006100010
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可