ヒトおよび環境から分離されたコレラ菌のｉｎ　ｖｉｔｒｏにおけるコレラ毒素産生性

• 山口 和美

  大阪薬科大学

• 西川 禎一

  大阪市立環境科学研究所

• 来住 輝彦

  大阪市立環境科学研究所

書誌事項

タイトル別名

• In vitro production of cholera toxin by Vibrio cholerae El Tor strains from human beings and the environment.

抄録

The present study was undertaken to evaluate culture media for cholera toxin production by Vibrio cholerae El Tor and compare enterotoxigenicity among isolates from humans and the environment. A total of 20 strains of V. cholerae were cultured in syncase medium, CAYE medium, yeast extract peptone water and AKI medium. The culture supernatant was assayed for cholera toxin by reversed passive latex agglutination. The AKI medium was found best for the in vitro production of cholera toxin. The organisms were cultured in AKI medium at 37C for 4hr in a stationary test tube and then for 16hr in tube continuously shaken. Of 11 isolates from patients and the vicinity in which they lived, 10 produced cholera toxin, while 9 isolates from river water failed to do so.

収録刊行物

• 生活衛生

  生活衛生 32 (2), 71-74, 1988

  社団法人 大阪生活衛生協会

詳細情報

• CRID: 1390001204207201664
• NII論文ID: 130003723618
• DOI: 10.11468/seikatsueisei1957.32.71
• ISSN: 18836631
• ISSN: 05824176
• Web Site: https://search.jamas.or.jp/link/ui/1989121234
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可