Characterization of cDNA clone encoding the matrix metalloproteinase 2 from rainbow trout fibroblast.

• SAITO MASATAKA

  Kagawa Nutrition College Laboratory of Marine Biochemistry, Tokyo University of Fisheries

• KUNISAKI NAOMICHI

  Kagawa Nutrition College

• URANO NAOTO

  Laboratory of Marine Biochemistry, Tokyo University of Fisheries

• KIMURA SHIGERU

  Laboratory of Marine Biochemistry, Tokyo University of Fisheries

抄録

Matrix metalloproteinases (MMP) are widely distributed in vertebrate tissues. One of the well-characterized gelatinases from higher vertebrates is MMP-2, named gelatinase A or 72 kDa type IV collagenase, which is active for the cleavage of denatured collagens, type IV collagen, type V collagen, and other matrix proteins. To investigate the primary structure and properties of MMP-2 from teleost as lower vertebrates, a cDNA library was prepared from mRNA of rainbow trout fibroblast and screened. Using polymerase chain reaction and degenerate oligonucleotide primers, which are specific for two highly conserved sequences found in MMP of higher vertebrates, a resultant cDNA fragment was used as a probe. A cDNA clone 3.0 kb long was isolated and found to contain an open-reading frame coding for a polypeptide of 655 amino acids. The rainbow trout polypeptide was 73% identical, at the level of amino acid sequence, to human proMMP-2 with the greatest degree of similarity occurring in the propeptide and catalytic domains and was denoted as rainbow trout proMMP-2. Then the isolated cDNA was expressed in Escherichia coli and the recombinant protein was found to degrade gelatin and human type V collagen, providing support to the hypothesis that the cDNA codes for the authentic rainbow trout proMMP-2. In contrast to human proMMP-2, rainbow trout proMMP-2 was not activated by 4-amino-phenylmercuric acetate. This is the first report of cDNA for fish proMMP to our knowledge.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 66 (2), 334-342, 2000

  公益社団法人 日本水産学会

キーワード

• amino acid sequence
• cDNA
• collagenase
• fibroblast
• fish
• gelatinase
• MMP-2
• rainbow trout

詳細情報

• CRID: 1390282679405054848
• NII論文ID: 130003742253
• NII論文ID: 30014677621
• NII論文ID: 10004858126
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2000.00052.x
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 5384312
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5384312
• 本文言語コード: en
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