Immunohistochemical Characterization of Monoclonal Antibodies (PDs) as Markers of the Periderm in the Developing Chicken Embryo.
-
- 磯川 桂太郎
- Division of Electron Microscopy, Dental Research Center, Nihon University Department of Anatomy, Nihon University School of Dentistry
-
- 本間 淳一
- Department of Anatomy, Nihon University School of Dentistry
-
- 村上 慶太
- Department of Anatomy, Nihon University School of Dentistry
-
- 田中 瑞穂
- Department of Anatomy, Nihon University School of Dentistry
-
- 青木 秀啓
- Department of Anatomy, Nihon University School of Dentistry
-
- 高橋 秀行
- Department of Anatomy, Nihon University School of Dentistry
-
- 横山 弘一
- Department of Anatomy, Nihon University School of Dentistry
-
- 遠藤 精一
- Department of Anatomy, Nihon University School of Dentistry
-
- 戸田 善久
- Division of Electron Microscopy, Dental Research Center, Nihon University Department of Anatomy, Nihon University School of Dentistry
抄録
The outermost surface cell layer of the developing embryo, the periderm, arises from the initial single layer of ectoderm and is eventually exfoliated from the stratified epidermis, which has the same ectodermal origin. In this study, monoclonal antibodies against chicken limb bud ectoderm were generated and screened for those which stained the periderm. Four separate antibodies termed PD2, 3, 7 and 9 were obtained from 180 mixed hybridomas. These PD antibodies stained the periderm selectively at all stages examined (stage 20-42). By correlating the results of immunohistochemistry with observations made by transmission electron microscopy, it was revealed that PD antibodies stained both the squamous periderm at an early stage and rounded bulging peridermal cells just before exfoliation. Therefore we feel that PD antibodies may be useful in further systematic investigations of the development and function of the chicken embryonic periderm.
収録刊行物
-
- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｎｉｈｏｎ Ｕｎｉｖｅｒｓｉｔｙ Ｓｃｈｏｏｌ ｏｆ Ｄｅｎｔｉｓｔｒｙ
-
Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｎｉｈｏｎ Ｕｎｉｖｅｒｓｉｔｙ Ｓｃｈｏｏｌ ｏｆ Ｄｅｎｔｉｓｔｒｙ 38 (3/4), 131-140, 1996
日本大学歯学部