Metabolism of 4-dimethylaminoazobenzene and related compounds,pt.2

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  • Metabolism of 4-Dimethylaminoazobenzene and Related Compounds. II. Metabolites of 4-Dimethylaminoazobenzene and 4-Aminoazobenzene in Rat Urine.

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The urinary metabolites of p-dimethylaminoazobenzene (DAB) and p-aminoazobenzene (AB) in a rat were examined. AB, ethereal sulfates of 4'-hydroxy-AB, N-acetyl-4'-hydroxy-AB, and 3-hydroxy-AB, p-acetamidoacetanilide, and conjugated forms of p-and o-aminophenol were detected from the AB-urine. As a new type of metabolite, ethereal sulfate of 3, 4'-dihydroxy-AB was also detected from the urine. These metabolites were also detected from the DAB-urine, in which the presence of ethereal sulfates of 4'-hydroxy-4-methylaminoazobenzene was also assumed. For the identification of these metabolites, several sulfates and glucuronides of aminoazo dyes were newly prepared and their physical properties were examined.

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