Biochemical Studies on the Metabolism of Penicillium islandicum Sopp. II. Acetyl-CoA Carboxylase and Related Enzymes

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In order to disclose the enzymatic mechanism of the biosynthesis of anthraquinoid pigment of P. islandicum Sopp. an investigation was made on acetyl-CoA carboxylase and related enzymes with the following results. 1) With the stationary culture of P. islandicum Sopp. on Czapek medium, luteoskyrin and other quinoid pigments were produced in the late stage of fungal growth ; 2) Sodium arsenate, monoiodoacetate, 2, 4-dinitrophenol and monofluoroacetate inhibited the fungal growth and the pigment formation, and malonate stimulated the latter. 3) With the crude and partially purified enzyme preparations of mechanically-disruptured mycelium, the formation of malonyl-CoA, the first intermediate of anthraquinoid pigments, was demonstrated. 4) With the crude extract of the mycelium, malonyl-CoA was decarboxylated to yield acetyl-CoA. 5) With the supernatant of the crude extract, the carboxyl group of pyruvate was exchanged with 14CO2. 6) In the early phase of the fungal growth, activities of aldolase and citrate synthetase were relatively high, and in the later stage activities of malate dehydrogenase and glucose-6-phosphate dehydrogenase were remarkably high.

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