Studies on the Passage of α-Chymotrypsin across the Intestine. II. Enzymic Activity and Radioactive Macromolecule recovered in the Mesenteric Perfusate
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A perfusion with Krebs-Ringer solution containing dextran through the mesenteric vascular system was performed on rat to which 131I-labelled α-chymotrypsin was administered in the jejunum. This method sufficed for the purpose of eliminating various blood components, such as the inhibitors for the enzyme, which interfer with quantitative assay of a small amount of the enzyme absorbed. Using the method, the non-diffusible 131I-bound substance (s) and N-acetyl-L-tyrosine ethyl ester (ATEE)-hydrolyzing activity were found in the mesenteric perfusates of the rats, and the amount of the enzyme absorbed during 90 min was estimated to be about 0.6% of the infused dose. Furthermore, when the immunoreactivity with the antiserum against α-chymotrypsin was examined on the mesenteric perfusate, a precipitin band sharply indicating the enzyme was formed with the perfusate collected during the first 30 min after the administration. These results strongly suggest that small but significant amount of α-chymotrypsin is transmitted across the intestinal wall into the mesenteric vascular system as retaining its enzymic and immunochemical properties.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 22 (5), 1029-1034, 1974
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204177807360
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- NII論文ID
- 110003654651
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- PubMed
- 4213971
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可