Studies on transfer ribonucleic acids and related compounds. X. Synthesis of the yeast tyrosine tRNA 5'-terminal oligonucleotides.

• 大塚 栄子

• 藤山 和男

• 大橋 稔

• 池原 森男

抄録

The pentanucleotide which has the sequence of 8th to 12th base of the 5'-terminus of yeast tyrosine transfer ribonucleic acid (tRNA), UpApm²GpCpC (1) has been synthesized by stepwise addition of properly protected mononucleotides using dicyolohexylcarbodiimide. 5'-O-Monomethoxytrityl-N²-methylguanosine 3'-phosphate was prepared by phosphorylation of the 5'-protected nucleoside to give the 2', 3'-cyclic phosphate and successive hydrolysis with ribonuclease (RNase) St. The isolated yields of the protected ditri, tetra-, and penta-nucleotides were 75, 38, 31, and 21%, respectively. The 5'-terminal tetranucleotide CpUpCpUp (3) corresponding to 1st to 4th nucleotides, was synthesized by condensation of the dinucleotide, Bz-C^Bz (OBz)-pU (OBz)-p (14) and the anilidate, C^Bz (OBz)-pU-(OBz)-pNHPh (16b) in a yield of 22%. The trinucleotide CpGpGp (2) spanning 5th to 7th nucleotides from the 5'-end was also prepared by the amidate method from the dinucleotide, MMTrC^Bz (OBz)-pG^Bu (OiBu)-p(18) and G^1Bu (OiBu)-pNHPhOCH₃ (19b) in a yield of 17%.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 24 (4), 570-579, 1976

  公益社団法人 日本薬学会

キーワード

• Drug Discovery
• General Chemistry
• General Medicine

詳細情報

• CRID: 1390282679149442688
• NII論文ID: 110003635016
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.24.570
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 779988
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/24/4/24_4_570/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1977066136
• 本文言語コード: en
• データソース種別:
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