A simple fluorescent post-labeling technique with o-phthalaldehyde for the analysis of proteins by polyacrylamide gel electrophoresis.
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A new polyacrylamide gel electrophoretic system with post-labeling of proteins with o-phthalaldehyde (OPT) has been developed. In the present system, the widely used tris, glycine and ammonium persulfate were replaced by triethanolamine, L-hydroxyproline and sodium persulfate, respectively. Proteins were visualized by dipping the gels in the OPT reagent for several minutes immediately after electrophoresis, and the gels were then scanned in a fluorescence densitometer. Fifty nanograms of bovine serum albumin could be detected by this technique.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 28 (12), 3696-3698, 1980
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679141157120
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- NII論文ID
- 110003623903
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可