Studies on immunological assay of urinary estrogens. IV. Comparison of the latex agglutination inhibition reaction method with gas chromatography-mass spectrometry.

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Estrogen levels in the urine of women at various stages of pregnancy were determined semi-quantitatively and quantitatively by the latex agglutination inhibition reaction (LAIR) method. Quantitative processing according to the LAIR method was possible by strictly standardizing the volume of the sample (30 μl) and latex reagents (35 μl), and the rotating speed of the glass slide (30 rpm). The values obtained by the LAIR method were compared with those by gas chromatography-mass spectrometry-selected ion monitoring (GC-MS-SIM) in which deutrated estriol (estriol-2, 4-d2) was used as an internal standard. There was a significant correlation between these values. The correlation coefficient was 0.9865 and the regression line was expressed as y=1.082x-2.10. The results indicate that the estrogen values semi-quantitatively determined by the LAIR method are reliable ones and that this procedure can be used effectively to determine estrogen levels in four ranges, less than 5 μg/ml, 5-10 μg/ml, 10-20 μg/ml, and more than 20 μg/ml, as a clinical screening test for monitoring feto-placental functions. The LAIR method is suitable for routine measurement of urinary estrogens.

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