Assay for pyrimidine nucleoside phosphorylases in tissue extracts by high-performance liquid chromatography.

• 河野 彬

• 菅田 節朗

• 原 泰寛

• 田中 睦子

• 加留部 喜晴

• 松島 美一

抄録

A sensitive method for the assay of pyrimidine nucleoside phosphorylases in preparations of human and animal tissues by determination of pyrimidines is described. Pyrimidines formed enzymatically from thymidine, uridine, 5-fluorouridine, and 5'-deoxy-5-fluorouridine are determined by means of high-performance liquid chromatography with ultraviolet (UV) detection. The pyrimidines, after extraction with ethyl acetate, are separated by reversed-phase chromatography on μ-Bondapak C-18/Porasil. The limits of detection are 2.5, 1.0, and 2.0 pmol for thymine, uracil, and 5-fluorouracil, respectively.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 31 (5), 1666-1669, 1983

  公益社団法人 日本薬学会

キーワード

• HPLC.

詳細情報

• CRID: 1390282679139442944
• NII論文ID: 130003769270
• DOI: 10.1248/cpb.31.1666
• ISSN: 13475223
• ISSN: 00092363
• PubMed: 6616714
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/31/5/31_5_1666/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可