Studies on transfer ribonucleic acids and related compounds. XLIV. A large-scale synthesis of the anticodon heptanucleotide of formyl-methionine transfer ribonucleic acid by using 2'-O-tetrahydrofuranylnucleosides.

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2'-O-Tetrahydrofuranyl nucleosides were prepared by an improved procedure via 3', 5'-tetraisopropyldisiloxanylnucleosides. A large scale synthesis of the anticodon heptanucleotide C-U-C-A-U-A-A of E. coli formylmethionine tRNA was performed by the phosphotriester method involving condensation of oligonucleotides having 2'-O-tetrahydrofuranyl protecting groups.

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