High-performance liquid chromatographic determination of 3-methylcytosine in deoxyribonucleic acid treated with carcinogenic methylating agents in vitro and in vivo.
抄録
A high-performance liquid chromatographic method is described for the determination of 3-methylcytosine (m3Cyt) in deoxyribonucleic acid (DNA). Calf thymus DNA was methylated with N-methyl-N-nitrosourea or N-methyl-N'-nitro-N-nitrosoguanidine in vitro. Methylated rat liver DNA was obtained by administration of dimethylnitrosamine to rats. The DNA samples were hydrolyzed with perchloric acid at 100°C for 1 h, and after the removal of perchloric acid by the use of anion exchange resin, the filtrate was concentrated and loaded on a Dowex 1×2 anion exchanger. The eluted cytosine derivatives were collected and injected into a liquid chromatograph. The content of m3Cyt in methylated DNA was successfully determined by this method. The amount of m3Cyt increased with the dosage of methylating agent in vitro, but showed no significant dose-dependent increase in vivo.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 33 (3), 1170-1174, 1985
公益社団法人 日本薬学会