ビオチンを利用したアビジン認識高分子膜

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タイトル別名
  • Avidin Identification with Polymer Membrane-Bound Biotin
  • ビチオン オ リヨウシタ アビジン ニンシキ コウブンシ マク

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抄録

Cellulose acetate membranes were modified with biotin to identify avidin. The follawing techniques were used to attach biotin to the surface of cellulose membranes. 1) Cellulose-chitosan composite cross-linked by glutaraldehyde, 2) Surface modification with polynuclear carboxylate metal complex formation, and 3) Surface modification with glycidylmethacrylate. Avidin identification was performed with these membranes. Every biotinylate membrane formed the complex with avidin on the surface, which resulted in membrane potential change. The extent of membrane potential change across cellulose membranes markedly depended on the charge density of the membrane matrix and on the charge change due to biotin-avidin complex formation.

収録刊行物

  • 高分子論文集

    高分子論文集 39 (10), 587-592, 1982

    公益社団法人 高分子学会

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