ピリミジン塩基を含む樹脂によるヌクレオシドの吸着クロマトグラフィー
書誌事項
- タイトル別名
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- Adsorption Chromatography of Cross-Linked Polystyrene Containing Pyrimidine Bases.
抄録
Cross-linked polystyrene containing nucleic acid bases such as uracil (U) or thymine (T) was prepared. The purpose was to separate adenosine (A) from guanosine (G) in mixed solutions of A and G by adsorption chromatography. The adsorption isotherms of A proved that A is chemically adsorped in the resin. The experimental conditions which show higher selectivity for A were surveyed by column method. No effect of space velocity was found. The resins of lower degree of cross-linking, in which the content of U is higher, showed higher amounts of adsorption of nucleoside per content of U in the resin; the resin of higher degree of cross-linking, however, showed higher selectivity for A. In the case of lower cross-linked resin, a higher relative ratio of adsorption (A/G) was obtained as the concentration of (A+G) solution decreases. In the case of higher cross-linked resin, a higher adsorption ratio (A/G) was obtained as the concentration of (A+G) increases. The fact that A was more adsorped than G in all cases suggests that A is hydrogen-bonded to U or T in the resin to form a base pair.
収録刊行物
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- 高分子論文集
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高分子論文集 49 (5), 419-425, 1992
公益社団法人 高分子学会
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詳細情報 詳細情報について
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- CRID
- 1390282681499719296
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- NII論文ID
- 130003837350
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- ISSN
- 18815685
- 03862186
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可