体外受精に由来する透明帯除去マウス胚の培養および移植成績

• 鈴木 宏志

  Drug Development Laboratories, Chugai Pharmaceutical Co., Ltd.

• 富樫 守

  Drug Development Laboratories, Chugai Pharmaceutical Co., Ltd.

• 宮井 達也

  Drug Development Laboratories, Chugai Pharmaceutical Co., Ltd.

• 岡本 道生

  Drug Development Laboratories, Chugai Pharmaceutical Co., Ltd.

書誌事項

タイトル別名

• Viability of zona- free mouse embryos der ived from eggs fertilized in vitro

抄録

The aim of the present study was to determine the potential for the further developmentof zona-free mouse zygotes fertilized in vitro. Zona-pellucida of (C57BL/6J X C3H/HeN) F1 mouse eggs-were removed with 0.5% pronase 6 hr after insemination. Each one of zonafreeeggs were cultured for 114 hr in 20μ1 medium in Multiplate-Terasaki and 20μl and 100pl drops in dish. At 96 hr after insemination 58%, 74% and 66% of these eggs, respectively, developed into the blastocyst stage. After transfer to the uterus of foster-mothers, 4 (5%) of 76 blastocysts cultured in 20μl medium in Multiplate-Terasaki, developed into newborns.It was concluded that the zona-pellucida is not essential for development into the youngfrom the zygotes fertilized in vitro.

収録刊行物

• 哺乳動物卵子研究会誌

  哺乳動物卵子研究会誌 4 (2), 84-91, 1987

  日本哺乳動物卵子学会

詳細情報

• CRID: 1390282679315859328
• NII論文ID: 130003839313
• DOI: 10.14893/jmor1984.4.84
• ISSN: 18846505
• ISSN: 02895439
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可