Complete mitochondrial DNA sequence of ayu Plecoglossus altivelis.

• ISHIGURO NAOYA

  Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University Ocean Research Institute, University of Tokyo

• MIYA MASAKI

  Department of Zoology, Natural History Museum & Institute

• NISHIDA MUTSUMI

  Ocean Research Institute, University of Tokyo

抄録

We determined the complete nucleotide sequence of the mitochondrial genome for ayu, Plecoglossus altivelis. Two large DNA fragments covering the entire genome were amplified using a long polymerase chain reaction (PCR) technique, and the products subsequently used as templates for PCR with 57 fish-versatile and five species-specific primers that amplify contiguous, overlapping segments of the entire genome. Direct sequencing of the PCR products demonstrated that the genome (16 537 bp) contained the same 37 mitochondrial genes (two ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes) as those found in other vertebrates, with the gene order identical to that in typical vertebrates. A major non-coding region between the tRNA^Pro and tRNA^Phe genes (857 bp) was considered to be the control region (D-loop), as it has several conservative blocks that are characteristic to this region.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 67 (3), 474-481, 2001

  公益社団法人 日本水産学会

キーワード

• ayu
• complete mitochondrial DNA
• long poymerization chain reaction
• mitogenomics
• Osmeriformes

詳細情報

• CRID: 1390001204428030592
• NII論文ID: 10008269297
• NII論文ID: 130003903573
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2001.00283.x
• COI: 1:CAS:528:DC%2BD3MXltVKjsb4%3D
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 5813245
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5813245
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可