卵巣腫ようにおけるＥｐｉｔｈｅｌｉａｌ　Ｍｅｍｂｒａｎｅ　Ａｎｔｉｇｅｎ（ＥＭＡ）の免疫細胞組織化学的検討

• 藤本 郁野

  癌研究会附属病院婦人科

• 荷見 勝彦

  癌研究会附属病院婦人科

• 増淵 一正

  癌研究会附属病院婦人科

• 長村 義之

  東海大学医学部病理学教室

• 渡辺 慶一

  東海大学医学部病理学教室

書誌事項

タイトル別名

• Cytochemical & histochemical study on epithelial membrane antigen(EMA) using immunoperoxidase technique in ovarian tumors.

抄録

Using indirect immunoperoxidase technique on histological sections and cytologic smears of peritoneal fluid from ovarian epithelial tumors, we have evaluated the role of Epithelial Membrane Antigen (EMA) and its association with histopathologic types and staining pattern related to histologic grade.<BR>Sections from 72 tissues (serous cystadenocarcinomas 37, mucinous cystadenocarcinomas 18, serous cystadenomas (LPM) 2, mucinous cystadenomas (LPM) 4, serous and mucinous cystadenomas 8 and normal ovaries 3) and the peritoneal smears from 18 serous cystadenocarcinomas and 10 mucinous cystadenocarcinomas were examined by immunohistochemical staining for EMA.<BR>The following results were obtained:<BR>1. In serous and mucinous tumors, the highest positive rate of EMA staining was observed in malignant groups, the second positive rate was in “LPM” groups, and lowest in benign groups.<BR>2. All of 37 serous cystadenocarcinomas was stained for EMA (100%), and of 18 mucinous cystadenocarcinomas 14 cases were stained for EMA (77.8%).<BR>3. In tissue specimens, EMA positive area was localized at luminal membranes in well differentiated adenocarcinomas, and in poorly differentiated, positive area was in diffuse cytoplasm.<BR>4. Comparing EMA-positive rate with CEA, hkp and CAl25 in serous cystadenocarcinomas, the higher positive rate of EMA and CAl25 was obtained than that of CEA and hkp. In contrast the positive rate of CEA in mucinous cystadenocarcinomas was higher than that of EMA.<BR>5. In cytological specimens, EMA staining was seenstrongly on entire cell membrane or diffuse cytoplasm. These findings were well correlated with those of histologic sections.<BR>These results suggest that immunocytochemical staining for EMA could be of value not only for the diagnosis of malignant ovarian tumors but also for the automated screening of peritoneal smears.

収録刊行物

• 日本臨床細胞学会雑誌

  日本臨床細胞学会雑誌 26 (6), 1019-1026, 1987

  公益社団法人　日本臨床細胞学会

キーワード

• Ovarian tumor
• Epithelial membrane antigen (EMA)
• Immunoperoxidase technique
• Cytology

詳細情報

• CRID: 1390282679670081024
• NII論文ID: 130003925734
• DOI: 10.5795/jjscc.26.1019
• ISSN: 18827233
• ISSN: 03871193
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可