QUANTITATIVE STUDIES ON THE INTERACTIONS BETWEEN HERPES SIMPLEX VIRUS AND ECTODERMAL CELL OF CHORIOALLANTOIC MEMBRANE OF FERTILE HEN'S EGG BY MEANS OF THE COVER SLIP INFECTION SYSTEM
書誌事項
- タイトル別名
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- I. ADSORPTION OF VIRUS ONTO CELLS
抄録
Elucidation of the mechanism of interactions between viruses and host cells has been advanced for the last decade most extensively in the studies on bacterial viruses. When similar investigations have been undertaken with animal viruses, there has been encountered the lack of an adequate host-virus system comparable to that of the bacterial virus from the viewpoint of exact quantitation. The basic conditions to be supplied by such host-virus system are (a) that host cells involved are uniformly susceptible to viral infection and react with the virus uniformly, (b) that for all virus particles there is an equal chance of access to cells, (c) that any desired number of virus particles can be brought in contact with known number of cells so as to adjust multiplicity of infection and finally (d) that the contact between virus and cells can be disrupted at any desired time by some appropriate means.<BR>Various attempts have been made to meet these criteria. First, the allantoic cavity of the developing chick embryo, being lined exclusively by entodermal cells, has been widely utilized for host-virus quantitation of influenza (reference in Henle, 1953) and other viruses (Sigel et al., 1951; Gordon et al., 1952) . Deembryonation technic more satisfactorily eliminated other tissues than entodermal cells from the system (Bernkopf, 1950; Finter et al., 1954) . On the other hand, entodermal cells of CAM (chorioallantoic membrane) of chick embryo have been utilized for similar studies on vaccinia (Briody and Stannard, 1951; Lépine et al., 1951; Oya, 1955) and herpes simplex viruses (Scott et al., 1953; Wildy, 1954; Modi and Tobin, 1954) . Meanwhile, Scherer (1953) succeeded in propagating herpes simplex and pseudorabies viruses in tissue culture with Earle's pure strain of cells, and Dulbecco and Vogt (1954a) demonstrated that tissue culture of monkey kidney and testis showed uniform susceptibility to poliomyelitis viruses. Weiss and Huang (1954) utilized in tissue culture chick embryo cells uniformly susceptible for feline and murine pneumonitis viruses.<BR>To increase the chance of collision and regulate multiplicity between virus and cells, suspensions of single cells were obtained by the enzymic digestion of cells (Scherer, Syverton and Gey, 1953; Dulbecco and Vogt, 1954b) . Other investigators have made use of Ehrlich ascites carcinoma and other tumor cells (Ackermann and Kurtz, 1952; Koprowska and Koprowski, 1953; Moore and Diamond, 1953), but the technical limitation for quantitation of this system lies in the short survival of the tumor cells in vitro.<BR>In the meantime, the technical ease and high sensitivity with which pock counting titration of herpes simplex virus can be done led the present authors to the attempt to improve the regulation of multiplicity in the herpes virusectodermal cell system given by Scott et al. (1953), by utilizing the cover slip technic to be reported here. Although this method requires further refinement in the future new information on the mode of adsorption onto and multiplication within CAM cells of herpes virus has been derived from the use of this system which will be reported in this and subsequent papers (Yoshino and Taniguchi, 1956) .
収録刊行物
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- Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｂｉｏｌｏｇｙ
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Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ ａｎｄ Ｂｉｏｌｏｇｙ 9 (6), 303-319, 1956
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会