破骨細胞（ｏｓｔｅｏｃｌａｓｔｓ）に関する研究　　培養骨髄細胞中の破骨細胞の同定とその形態学的特徴

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  • Studies on osteoclasts. Identification and characterization of osteoclasts in bone marrow cell culture.
  • Identification and characterization of osteoclasts in bone marrow cell culture
  • 培養骨髄細胞中の破骨細胞の同定とその形態学的特徴

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In order to identify and characterize osteoclasts morphologically, bone marrow cells were isolated from the long bones of neonatal rats and suspended in Hepes-buffered 199 medium containing 0.1% bovine serum albumin. The cell suspensions were plated on coverslips settled in petri dishes and incubated at 37°C in a humidified air containing 5% CO2. At intervals after incubation, the adherent cells were stained with May-Gruwald-Giemsa solution and then observed morphologically under the microscope.<BR>In this bone marrow culture, osteoclasts were identified as multinucleated giant cells, and could be classified into two different types by the characteristic morphology of their cytoplasm: the cells with a cytoplasm showing only typical lamellipodia (L-type), those with a lamellipodia, lobopodia, polarity and ruffling activity (P-type).<BR>Almost all osteoclasts which were cultured by this method were dead within 24 hours.

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